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Protein G Magnetic Beads
Protein G Magnetic Beads from Cell Signaling Technology
Be the first to write a review!
Citations:
(316)
Supplier Page
Supplier Page from
Cell Signaling Technology for
Protein G Magnetic Beads
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Protein G Magnetic Beads
Company
Cell Signaling Technology
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
70024S
Quantity
1 ml
Applications
IP
Research Area
Related Products
Reactivity
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Type
Magnetic Beads
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Cell Signaling Technology
3 Trask Lane
Danvers
,
Massachusetts
01923
United States
Phone
: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488
Company Profile
Website:
http://www.cellsignal.com/
Website:
Technical Support
Citations (316)
(1)
Oncogenic DNA methyltransferase 1 activates the PI3K/AKT/mTOR signalling by blocking the binding of HSPB8 and BAG3 in melanoma
Epigenetics
December 31, 2023
Yemei Yang, Shengfang Ma, Zi Ye, Yushi Zheng, Zhenjiong Zheng, Xiongshan Liu, Xianyi Zhou
5’-methylcytosine antibody (
ab10805
,
Abcam
) and IgG antibody (#
14705
,
CST
), respectively. After that, 50 μL of
protein G magnetic beads
was re-added for 3-h
incubation
and washed with 1× IP buffer, followed by 2-h
incubation
with proteinase
(2)
TRIM21-mediated Sohlh2 ubiquitination suppresses M2 macrophage polarization and progression of triple-negative breast cancer
Cell Death Dis
December 20, 2023
Ruihong Zhang, Ying Shen, Qi Zhang, Xiaoning Feng, Xuyue Liu, Xiaoning Huo, Jinhao Sun, Jing Hao
chromatin
fragments solutions were incubated with 10 µl anti-Sohlh2 antibody or the rabbit IgG and
Protein G magnetic beads
at 4 °C overnight with rotation state. The precipitated DNAs were eluted, separated by magnetic separator,
(3)
Identification and partial characterization of new cell density-dependent nucleocytoplasmic shuttling proteins and open chromatin
Sci Rep
December 8, 2023
Kangjing Li, Yaxin Li, Fumihiko Nakamura
technical negative control,
Cell Signaling Technologies
) overnight with rotation, then incubated with 30 μL
Protein G Magnetic Beads
for 2 h with rotation in accordance with SimpleChIP Enzymatic
Chromatin
IP kit (Cell Signaling Technologies)
(4)
Affinity-tagged SMAD1 and SMAD5 mouse lines reveal transcriptional reprogramming mechanisms during early pregnancy
bioRxiv
December 5, 2023
Zian Liao, Suni Tang, Kaori Nozawa, Keisuke Shimada, Masahito Ikawa, Diana Monsivais, Martin M. Matzuk
C29F4) or PA antibody (Fuji Film, NZ-1) to the lysate and incubate for 1 hour at 4 °C. Subsequently,
protein G magnetic beads
(
Thermo Fisher
,
88847
) were added for an additional 1 hour at 4 °C. Then, the beads were washed five
(5)
Mitochondrial permeability transition regulator, cyclophilin D, is transcriptionally activated by C/EBP during adipogenesis
Journal of Biological Chemistry
December 1, 2023
Chen Yu, Rubens Sautchuk, John Martinez, Roman A. Eliseev
antibody (Cell Signaling,
8178
,
1:50
) or control nonimmune IgG. Immunocomplexes were then purified with
protein G magnetic beads
(
Cell Signaling
,
9006
), washed, and resuspended in 2x Laemmli buffer.
Western blot analysis
was performed
(6)
ANGPTL2-mediated epigenetic repression of MHC-I in tumor cells accelerates tumor immune evasion
Molecular oncology
December 1, 2023
Kadomatsu T, Hara C, Kurahashi R, Horiguchi H, Morinaga J, Miyata K, Kurano S, Kanemaru H, Fukushima S, Araki K,
et al
.
(#
2729
;
Cell Signaling Technology
) overnight at 4 °C.
Chromatin
complexes were then precipitated with
Protein G magnetic beads
(
Cell Signaling Technology
). After washing, DNA was purified from samples and subjected to
real‐time PCR
(7)
Inhibition of PKA/CREB1 pathway confers sensitivity to ferroptosis in non-small cell lung cancer
Respiratory Research
November 13, 2023
Guangyao Shan, Guoshu Bi, Guangyin Zhao, Jiaqi Liang, Yunyi Bian, Huan Zhang, Xing Jin, Zhengyang Hu, Guangyu Yao, Hong Fan,
et al.
antibody (
1:50
, #
9197
,
Cell Signaling Technology
) was added. After the complex was co-precipitated,
Protein G magnetic beads
were exploited to capture the complex. The cross-links were then reversed after the
chromatin
was eluted.
(8)
Germline cis variant determines epigenetic regulation of the anti-cancer drug metabolism gene dihydropyrimidine dehydrogenase (DPYD)
bioRxiv
November 9, 2023
Ting Zhang, Alisa Ambrodji, Huixing Huang, Kelly J. Bouchonville, Amy S. Etheridge, Remington E. Schmidt, Brianna M. Bembenek, Zoey B. Temesgen, Zhiquan Wang, Federico Innocenti,
et al.
normal Rabbit IgG antibody (antibody
2729
;
Cell Signaling Technology
, Danvers, MA), in the presence of
protein G magnetic beads
, ChIP buffer, and protease
inhibitor
cocktail (all Active Motif) for 4 hours at 4°C. Magnetic beads
(9)
The E301R protein of African swine fever virus functions as a sliding clamp involved in viral genome replication
mBio
October 31, 2023
Su Li, Hailiang Ge, Yanhua Li, Kehui Zhang, Shaoxiong Yu, Hongwei Cao, Yanjin Wang, Hao Deng, Jiaqi Li, Jingwen Dai,
et al.
no.
9003S
#
2729
,
Cell Signaling Technology
) antibodies for 4 hours at 4°C and then incubated with the
protein G magnetic beads
(catalog no.
9003S
#
9006
,
Cell Signaling Technology
) for 2 hours at 4°C. The beads were washed three
(10)
Intermittent Hypoxia Promotes TAM-Induced Glycolysis in Laryngeal Cancer Cells via Regulation of HK1 Expression through Activation of ZBTB10
International Journal of Molecular Sciences
September 30, 2023
Minlan Yang, Weisong Cai, Zehua Lin, Aikebaier Tuohuti, Xiong Chen
(#
2729
,
Cell Signaling Technology
). Samples were incubated at 4 °C with shaking overnight. Then, 30 μL of
Protein G Magnetic Beads
per sample was added the next day and incubated at 4 °C with shaking for 2 h. The Protein G Magnetic
(11)
METTL3‐mediated N6‐methyladenosine exacerbates ferroptosis via m6A‐IGF2BP2‐dependent mitochondrial metabolic reprogramming in sepsis‐induced acute lung injury
Clinical and Translational Medicine
September 15, 2023
Hao Zhang, Dan Wu, Yanghanzhao Wang, Kefang Guo, Charles B. Spencer, Lilibeth Ortoga, Mengdi Qu, Yuxin Shi, Yuwen Shao, Zhiping Wang,
et al.
immunoglobulin G (
2729
,
Cell Signaling Technology
) at 4°C for 12 h. The immunoprecipitate was bound to
protein G magnetic beads
, and DNA–protein crosslinking was terminated by
incubation
at 65°C for 2 h. Finally, the
immunoprecipitated
(12)
Butyrate reverses ferroptosis resistance in colorectal cancer by inducing c-Fos-dependent xCT suppression
Redox Biology
September 1, 2023
Ying He, Yuhang Ling, Zhiyong Zhang, Randall Tyler Mertens, Qian Cao, Xutao Xu, Ke Guo, Qian Shi, Xilin Zhang, Lixia Huo,
et al.
-
c-FOS rabbit mAb
(
Cell Signaling Technology
, Cat#
2250T
,
1:50
), or rabbit IgG at 4 °C overnight. Thereafter,
protein G magnetic beads
were added and a 2-h
incubation
with rotation was conducted. After
chromatin
elution and
DNA purification
,
(13)
P65 mediated UBR4 in exosomes derived from menstrual blood stromal cells to reduce endometrial fibrosis by regulating YAP Ubiquitination
Journal of Nanobiotechnology
August 29, 2023
Jiarui Qi, Xudong Zhang, Siwen Zhang, Shanshan Wu, Yimeng Lu, Shuyu Li, Pingping Li, Jichun Tan
the P65 antibody (#
8242
,
CST
, USA) antibodies were added and mixed overnight at 4℃.On the second day,
protein G magnetic beads
were added to collect the antibody-bound
chromatin
DNA complex. Then the DNA was washed and unlinked
(14)
Dual Targeting of Apoptotic and Signaling Pathways in T-Lineage Acute Lymphoblastic Leukemia
Clinical cancer research : an official journal of the American Association for Cancer Research
August 15, 2023
Saygin C, Giordano G, Shimamoto K, Eisfelder B, Thomas-Toth A, Venkataraman G, Ananthanarayanan V, Vincent TL, DuVall A, Patel AA,
et al
.
NF-κB p65 antibody
(D14E12,
Cell Signaling Technology
) at 4°C overnight. After
incubation
, 30 μL of
protein G magnetic beads
were added to immunoprecipitating complexes and incubated for 2 hours at 4°C. The immunoprecipitating
(15)
FOXA2 controls the anti-oxidant response in FH-deficient cells
Cell Reports
July 25, 2023
Connor Rogerson, Marco Sciacovelli, Lucas A. Maddalena, Andromachi Pouikli, Marc Segarra-Mondejar, Lorea Valcarcel-Jimenez, Christina Schmidt, Ming Yang, Elena Ivanova, Joshua Kent,
et al.
(
CST
2729S
) overnight at 4°C. The following day, the
chromatin
-antibody complexes were incubated with
Protein G magnetic beads
(
Invitrogen
10009D
), for 2 h at 4°C, rotating.
Chromatin
-antibody-bead complexes were washed twice with
(16)
The uncharacterized transcript KIAA0930 confers a cachexic phenotype on cancer cells
Oncotarget
July 20, 2023
Yamakawa T, Zhang G, Najjar LB, Li C, Itakura K.
(
F1804
;
Millipore Sigma
; Burlington, MA, USA) overnight at 4°C with gentle shaking. Thirty microliters of
Protein G Magnetic Beads
(#
9006
;
Cell Signaling Technology
) were then added, and the mixture was incubated for an additional
(17)
Nicotinamide enhances osteoblast differentiation through activation of the mitochondrial antioxidant defense system
Experimental & molecular medicine
July 1, 2023
Yoon H, Park SG, Kim HJ, Shin HR, Kim KT, Cho YD, Moon JI, Park MS, Kim WJ, Ryoo HM.
incubated overnight with anti-acetyl lysine antibody (
Cell Signaling Technology
, USA) on a rotator at 4 °C.
Protein G magnetic beads
were added to the supernatant and incubated for 2 h at 4 °C. These beads were then collected and washed
(18)
MET Receptor Tyrosine Kinase Inhibition Reduces Interferon-Gamma (IFN-γ)-Stimulated PD-L1 Expression through the STAT3 Pathway in Melanoma Cells
Cancers
June 29, 2023
Song KY, Han YH, Roehrich H, Brown ME, Torres-Cabala C, Giubellino A.
Immunoprecipitation (IP
)
Immunoprecipitation
procedures
were performed with
protein G magnetic beads
from
Cell Signaling
(Cat. #
70024
). All the cell lysates were pre-cleared with lysate buffers and 500 μg of cell lysates were used for
(19)
MiR-431 attenuates synaptic plasticity and memory deficits in APPswe/PS1dE9 mice
JCI insight
June 22, 2023
Ge J, Xue Z, Shu S, Yu L, Qin R, Tao W, Liu P, Dong X, Lan Z, Bao X,
et al
.
ChIP-Grade Protein G Magnetic Beads
were added and incubated for 2 hours. DNA was eluted from antibody/
Protein G Magnetic Beads
, and the cross-links were unlocked and purified using spin columns for
qPCR
. Sequences of primers for
(20)
YES1 Kinase Mediates the Membrane Removal of Rescued F508del-CFTR in Airway Cells by Promoting MAPK Pathway Activation via SHC1
Biomolecules
June 6, 2023
Patrícia Barros, Ana M. Matos, Paulo Matos, Peter Jordan
(#
65890
,
Cell Signaling
) at 4 °C with agitation. Then, protein-antibody complexes were incubated with
protein G magnetic beads
(Thermo Fisher Scientific, Waltham, MA, USA) for 1 h at 4 °C with agitation, and after five washes with
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Protein G Magnetic Beads from Cell Signaling Technology
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Pricing Info
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Company Info
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